ULTRON AF-HILIC-CD series

ULTRON AF-HILIC-CD series is a HILIC mode column in which β-cyclodextrin (※) is chemically bonded to a silica stationary phase via a spacer. The selectivity towards molecular structure is high, and also shows high resolution analysis for structural analogues and structural isomers of acidic, basic and neutral compounds.

（※） CD has a hallow cavity derived from its cyclic structure and are hydrophobic on the inside and hydrophilic on the outside. It is characterized by the inclusion complex formation that takes with various compounds by the intermolecular force inside the cyclic structure.

Hydrophilic Interaction Chromatography (HILIC) is a separation mode used for analysis of polar compounds that are difficult to retain in reversed phase column. Polar compounds elute later than hydrophobic compound. In case of analysis of hydrophilic compounds, ion pair reagents are often used in order to retain in reversed phase columns, but in HILIC it is possible to retain and separate without using this ion pair reagent.

Specification

Base material	Functional group	Particle size	Pore size	Surface area	pH range
High purity totally spherical porous silica gel	β-cyclodextrin	5µm	12nm	300m2/g	2-7
High purity totally spherical porous silica gel	β-cyclodextrin	2µm	12nm	300m2/g	2-7

Simultaneous analysis of mono-, di- and oligosaccharides

ULTRON AF-HILIC-CD series :Reproducibility

ULTRON AF-HILIC-CD series: Scalability (2 um, 5 um)

Comparison of Schiff base formation and anomer separation

Effect of mobile phase pH and buffer concentration on retention behavior

Separation of sugar isomers

High-throughput analysis of melamine related compounds

Simultaneous analysis of sugars and sweeteners