Hydrophilic Interaction Chromatography
The ULTRON AF-HILIC series is HILIC mode column. These columns are modified with highly polar groups that can retain polar compounds which are difficult to retain in reversed phase mode.
ULTRON AF-HILIC-CD is a HILIC mode column in which β-cyclodextrin (※) is chemically bonded to a silica stationary phase via a spacer. The selectivity towards molecular structure is high, and also shows high resolution analysis for structural analogues and structural isomers of acidic, basic and neutral compounds.
（※） CD has a hallow cavity derived from its cyclic structure and are hydrophobic on the inside and hydrophilic on the outside. It is characterized by the inclusion complex formation that takes with various compounds by the intermolecular force inside the cyclic structure.
Hydrophilic Interaction Chromatography (HILIC) is a separation mode used for analysis of polar compounds that are difficult to retain in reversed phase column. Polar compounds elute later than hydrophobic compound. In case of analysis of hydrophilic compounds, ion pair reagents are often used in order to retain in reversed phase columns, but in HILIC it is possible to retain and separate without using this ion pair reagent.
|Particle size||Pore size||Surface area||pH range|
|High purity totally spherical porous silica gel||β-cyclodextrin||5µm||12nm||300m2/g||2-7|
Simultaneous analysis of mono-, di- and oligosaccharides
ULTRON AF-HILIC-CD series :Reproducibility
ULTRON AF-HILIC-CD series: Scalability (2 um, 5 um)
Comparison of Schiff base formation and anomer separation
Effect of mobile phase pH and buffer concentration on retention behavior
Separation of sugar isomers
High-throughput analysis of melamine related compounds
Simultaneous analysis of sugars and sweeteners