LC
- Samples
- Cholic acid , Glycocholic acid , Taurocholic acid , Chenodeoxycholic acid , Glycochenodeoxycholic acid , Deoxycholic acid , Taurochenodeoxycholic acid , Taurodeoxycholic acid
- Applications
- Bile acid
- Column
- STR ODS-II
- Column Size
- 150 mm L × 4.6 mm I.D.
- Detector
- UV-217 nm
- Particle Size
- 5 µm
- Mobile phase
- CH3CN/30 mM (NH4)2HPO4(Ajusted to pH 7.2 with NH4H2PO4), A: 10/90, B: 50/50
- Frow rate
- 1.0 mL/min
- Gradient
-
Time Profile (min) %A %B 0 70 30 55 15 85 - Column temp.
- 40°C