Shinwa Chemical Industries Ltd.
LC/GC Search / Application Note

Water-soluble vitamins

Graph

LC

Samples
Nicotinamide , Pyridoxine , Isoascorbic acid , Riboflavin , Nicotinic acid , Ascorbic acid , Biotin , Pantothenic acid , Folic acid , Cyanocobalamin , Thiamine
Applications
Water-soluble vitamins
Column
ULTRON AF-HILIC-CD
Column Size
150 mm L × 2.0 mm I.D.
Detector
UV-254 nm
Particle Size
2 µm
Mobile phase
A: 20 mM CH3CO2NH4(pH 6.8)/CH3CN = 10/90, B: 20 mM CH3CO2NH4(pH 6.8)/CH3CN = 70/30
Frow rate
0.25 mL/min
Gradient
15 minutes gradient, 85% - 50% with 10 minutes re-equilibration
TimeProfile
(min)%A%B
0.00 91 9
5.00 33 67
15.00 33 67
15.01 91 9
25.00 91 9
Column temp.
30°C
Injection volume
1.0 µL

Price list

lc_00222