LC
- Samples
- Nicotinamide , Pyridoxine , Isoascorbic acid , Riboflavin , Nicotinic acid , Ascorbic acid , Biotin , Pantothenic acid , Folic acid , Cyanocobalamin , Thiamine
- Applications
- Water-soluble vitamins
- Column
- ULTRON AF-HILIC-CD
- Column Size
- 150 mm L × 2.0 mm I.D.
- Detector
- UV-254 nm
- Particle Size
- 2 µm
- Mobile phase
- A: 20 mM CH3CO2NH4(pH 6.8)/CH3CN = 10/90, B: 20 mM CH3CO2NH4(pH 6.8)/CH3CN = 70/30
- Frow rate
- 0.25 mL/min
- Gradient
- 15 minutes gradient, 85% - 50% with 10 minutes re-equilibration
Time Profile (min) %A %B 0.00 91 9 5.00 33 67 15.00 33 67 15.01 91 9 25.00 91 9 - Column temp.
- 30°C
- Injection volume
- 1.0 µL