Shinwa Chemical Industries Ltd.
LC/GC Search / Application Note

Analysis of melamine compounds

Graph

LC

Samples
Cyanuric acid , Ammelide , Melamine , Ammeline
Applications
Analysis of melamine compounds
Column
ULTRON AF-HILIC-CD
Column Size
100 mm L × 2.0 mm I.D.
Detector
UV-220 nm
Particle Size
2 µm
Mobile phase
20mM HCO2NH4 (pH6.8) /CH3CN = 18/82
Frow rate
0.15 mL/min
Column temp.
30°C
Injection volume
2.0 µL
Diluent
Dissolved samples in 0.1% NaOH aq and add acetonitrile into ratio to 50%

Cyanuric acid (R1= OH, R2= OH, R3= OH)
Ammelide (R1= OH, R2= OH, R3= NH2)
Melamine (R1= NH2, R2= NH2, R3= NH2)
Ammeline (R1= OH, R2= NH2, R3= NH2)

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