Shinwa Chemical Industries Ltd.
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Analysis of xanthines

Graph

LC

Samples
Caffeine , Theophylline , Theobromine , 1-Methylxanthine , Xanthine
Applications
Analysis of xanthines
Column
ULTRON AF-HILIC-CD
Column Size
150 mm L × 2.0 mm I.D.
Detector
UV-254 nm
Particle Size
2 µm
Mobile phase
10mM CH3CO2NH4 (pH6.8) /CH3CN = 13/87
Frow rate
0.15 mL/min
Column temp.
30°C
Injection volume
1.0 µL
Diluent
Dissolved samples in 0.1% NaOH aq and add acetonitrile into ratio to 90%

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