LC
- Samples
- Methotrexate
- Applications
- Analysis of Methotrexate
- Column
- ULTRON ES-BSA-2
- Column Size
- 150 mm L × 4.0 mm I.D.
- Detector
- UV-302 nm
- Particle Size
- 7 µm
- Mobile phase
- 50mM sodium phosphate buffer (pH6.9)/1-Propanol = 92/8
- Frow rate
- 1.5 mL/min
- Column temp.
- 25°C
- Injection volume
- 20.0 µL
- Diluent
- mobile phase
0.02 mg/mL each of USP Methotrexate RS and USP R- Methotrexate RS