LC
- Samples
- Oligo deoxythymidine primers , Oligo dT primers
- Applications
- Analyses of Oligo deoxythymidine primers
- Column
- ULTRON AF-HILIC-CD
- Column Size
- 150 mm L × 2.0 mm I.D.
- Detector
- UV-260 nm
- Particle Size
- 2 µm
- Mobile phase
- A: CH3CN B: 100 mM CH3CO2NH4 (pH 6.7)
- Frow rate
- 0.15 mL/min
- Gradient
- 27 minutes gradient, 30% - 42% with 13 minutes re-equilibration
Time Profile (min) %A %B 0.00 70 30 6.00 64 36 27.00 58 42 27.01 70 30 40.00 70 30 - Column temp.
- 30°C
- Injection volume
- 5.0 µL
- Diluent
- CH3CN/TE buffer* = 70/30
* TE buffer = 10 mM Tris-HCl buffer pH 8.0 + 1 mM EDTA buffer pH 8.0