LC
- Samples
- Oligo deoxythymidine primers, 6-25 mer
- Applications
- Analysis of Oligo-deoxythymidine using the ULTRON AF-HILIC-CD “Effect of the type of salt and ionic strength in the mobile phase”
- Column
- ULTRON AF-HILIC-CD
- Column Size
- 150 mm L × 2.0 mm I.D.
- Detector
- UV-260 nm
- Particle Size
- 2 µm
- Frow rate
- 0.15 mL/min
- Column temp.
- 30°C
- Injection volume
- 0.5 µL
- Diluent
- CH3CN/TE buffer* = 70/30
Mobile phase
1.
A) CH3CN
B) 20 mM CH3CO2NH4
30-33 %B(0-3 min), 33 %B(3-20 min), 30 %B(20-40 min)
2.
A) CH3CN
B) 50 mM CH3CO2NH4
30-36 %B(0-6 min), 36-40 %B(6-27min), 30 %B(27-40 min)
3.
A) CH3CN
B) 100 mM CH3CO2NH4
30-36 %B(0-6 min), 36-42 %B(6-27min), 30 %B(27-40 min)
4.
A) CH3CN/20 mM KH2PO4-K2HPO4(pH 6.5) = 70/30
B) CH3CN/20 mM KH2PO4-K2HPO4(pH 6.5) = 30/70
0-9 %B(0-8 min), 9-12 %B(8-30min), 0 %B(30-40 min)
* TE buffer = 10 mM Tris-HCl buffer pH 8.0 + 1 mM EDTA buffer pH 8.0