LC
- Samples
- Phosphorothioate oligo-deoxythymidine
- Applications
- Analysis of Phosphorothioate oligo-deoxythymidine using the ULTRON AF-HILIC-CD (Part 4) “Relationship between PS sites and retention time in dT20”
- Column
- ULTRON AF-HILIC-CD
- Column Size
- 150 mm L × 2.0 mm I.D.
- Detector
- UV-260 nm
- Particle Size
- 2 µm
- Mobile phase
- 100 mM CH3CO2NH4/CH3CN = 38/62
- Frow rate
- 0.15 mL/min
- Column temp.
- 30°C
- Injection volume
- 0.5 µL
- Diluent
- CH3CN/TE buffer※ = 70/30
※ TE buffer = 10 mM Tris-HCl buffer pH 8.0 + 1 mM EDTA buffer pH 8.0
Sample
1. 5'-TTTTTTTTTTTTTTTTTTTT-3’
2. 5'-T＊TTTTTTTTTTTTTTTTTTT-3’
3. 5'-TTTTTTTTTT＊TTTTTTTTTT-3’
4. 5'-TTTTTTTTTTTTTTTTTTT＊T-3’
5. 5'-T＊T＊T＊T＊T＊T＊T＊T＊T＊T＊T＊T＊T＊T＊T＊T＊T＊T＊T＊T-3’
“＊ …PS modification