LC
- Samples
- Universal primer T7
- Applications
- Analysis of Universal primer T7 using the ULTRON AF-HILIC-CD “Difference in retention time by addition of different alcohols to mobile phase”
- Column
- ULTRON AF-HILIC-CD
- Column Size
- 150 mm L × 2.0 mm I.D.
- Detector
- UV-260 nm
- Particle Size
- 2 µm
- Frow rate
- 0.15 mL/min
- Gradient
-
Time Profile (min) %A %B 0 65 35 60 50 50 60 75 35 75 75 35 - Column temp.
- 30°C
- Injection volume
- 0.5 µL
- Diluent
- CH3CN/TE buffer※ = 70/30
※ TE buffer = 10 mM Tris-HCl buffer pH 8.0 + 1 mM EDTA buffer pH 8.0
Mobile phase
A) CH3CN
B-1) 100 mM CH3CO2NH4 in Water
B-2) 100 mM CH3CO2NH4 in Water/Methanol = 90/10
B-3) 100 mM CH3CO2NH4 in Water/2-Propanol = 90/10
T7 : 5'-TAA TAC GAC TCA CTA TAG GG-3’