LC
- Samples
- Phosphorothioate oligo-deoxythymidine
- Applications
- Analysis of PSdT6 and [PHO]-PSdT6-[PHO] using the ULTRON AF-HILIC-CD (Part 2) “Effect of analysis temperature”
- Column
- ULTRON AF-HILIC-CD
- Column Size
- 150 mm L × 2.0 mm I.D.
- Detector
- UV-260 nm
- Particle Size
- 2 µm
- Mobile phase
- 200 mM CH3CO2NH4/CH3CN = 35/65
- Frow rate
- 0.15 mL/min
- Column temp.
- 10-50°C
- Injection volume
- 0.5 µL
- Diluent
- CH3CN/TE buffer※ = 70/30
※ TE buffer = 10 mM Tris-HCl buffer pH 8.0 + 1 mM EDTA buffer pH 8.0
Sample
1. 5'-T＊T＊T＊T＊T＊T-3’
2. 5'-[PHO]-T＊T＊T＊T＊T＊T-[PHO]-3’
“＊ …PS modification