LC

Samples

Val-Tyr-Val , Leucine Enkephalin , Methionine Enkephalin , Gly-Tyr

Applications

Analysis of peptide standards

Column

ULTRON AF-HILIC-CD

Column Size

150 mm L × 2.0 mm I.D.

Mobile phase

A: 10mM HCO2NH4 (pH3.0) /CH3CN = 10/90, B: 10mM HCO2NH4 (pH3.0) /CH3CN = 70/30

Frow rate

0.26 mL/min: 67% A/ 33% B (70.0% CH3CN)

Column temp.

40°C

Injection volume

2.0 µL

Diluent

H2O /CH3CN = 50/50

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