LC

Samples

Maleic acid , Ascorbic acid , Citric acid , Fumaric acid

Applications

Organic acid and vitamin C in the juice (other the maleic acid)

Column

ULTRON VX-ODS

Column Size

150 mm L × 4.6 mm I.D.

Mobile phase

20 mM Phosphoric acid buffer(pH 2.4)

Frow rate

0.8 mL/min

Column temp.

ambient

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LC

Samples

Nicotinamide , Pyridoxine , Isoascorbic acid , Riboflavin , Nicotinic acid , Ascorbic acid , Biotin , Pantothenic acid , Folic acid , Cyanocobalamin , Thiamine

Applications

Water-soluble vitamins

Column

ULTRON AF-HILIC-CD

Column Size

150 mm L × 2.0 mm I.D.

Mobile phase

A: 20 mM CH3CO2NH4(pH 6.8)/CH3CN = 10/90, B: 20 mM CH3CO2NH4(pH 6.8)/CH3CN = 70/30

Frow rate

0.25 mL/min

Column temp.

30°C

Injection volume

1.0 µL

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LC

Samples

Maleic acid , Pyruvic acid , Ascorbic acid , Gluconic acid , Adipic acid , Fumaric acid , Tartaric acid , Malic acid , Citric acid

Applications

Analysis of organic acid

Column

ULTRON AF-HILIC-CD (PLSS)

Column Size

150 mm L × 2.0 mm I.D.

Mobile phase

100mM CH3CO2NH4 (pH6.8) /CH3CN = 25/75

Frow rate

0.20 mL/min

Column temp.

40°C

Injection volume

2.0 µL

Diluent

mobile phase

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LC

Samples

Dehydroascorbic acid , Ascorbic acid

Applications

Analyses of Ascorbic acid

Column

ULTRON AF-HILIC-CD

Column Size

150 mm L × 2.0 mm I.D.

Mobile phase

100mM CH3CO2NH4 (pH6.8) /CH3CN = 30/70

Frow rate

0.2 mL/min

Column temp.

30°C

Injection volume

10.0 µL

Diluent

H2O /CH3CN = 10/90

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