LC

Samples

Nicotinic acid , Nicotinamide , Pantothenic acid , Pyridoxine , Riboflavin phosphate , Thiamine , Caffeine , Folic acid , Biotin , Riboflavin

Applications

The water-soluble vitamin (1)

Column

STR ODS-II

Column Size

150 mm L × 4.6 mm I.D.

Mobile phase

100 mM Phosphoric acid buffer(pH 2.1) + 0.8 mM Octane sodium sulphonate/CH3CN = 9/1

Frow rate

1.0 mL/min

Column temp.

40°C

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LC

Samples

Nicotinic acid , Nicotinic acid amide , Calcium pantothenate , Pyridoxine hydrochloride , Riboflavin phosphate , Thiamine , Folic acid , Biotin , Riboflavin

Applications

The water-soluble vitamin

Column

ULTRON VX-ODS

Column Size

150 mm L × 4.6 mm I.D.

Mobile phase

20 mM H3PO4/5 mM Sodium 1-hexanesulfonate = 91/9

Frow rate

1.0 mL/min

Column temp.

45°C

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LC

Samples

Nicotinamide , Pyridoxine , Isoascorbic acid , Riboflavin , Nicotinic acid , Ascorbic acid , Biotin , Pantothenic acid , Folic acid , Cyanocobalamin , Thiamine

Applications

Water-soluble vitamins

Column

ULTRON AF-HILIC-CD

Column Size

150 mm L × 2.0 mm I.D.

Mobile phase

A: 20 mM CH3CO2NH4(pH 6.8)/CH3CN = 10/90, B: 20 mM CH3CO2NH4(pH 6.8)/CH3CN = 70/30

Frow rate

0.25 mL/min

Column temp.

30°C

Injection volume

1.0 µL

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