LC

Samples

Aspartic acid , Glutamic acid , Serine , Glycine , Histidine , Arginine , Threonine , Alanine , Proline , Tyrosine , Valine , Methionine , Cysteine-cysteine , Isoleucine , Leucine , Phenylalanine , Lysine

Applications

Analysis of the PTC amino acid

Column

STR ODS-II

Column Size

150 mm L × 4.6 mm I.D.

Mobile phase

A: CH3COONa (pH 6.5)/CH3CN = 93/7, B: CH3CN/H2O = 4/1

Frow rate

1.0 mL/min

Column temp.

40°C

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LC

Samples

(CYS)2 , LYS , ARG , HIS , ASP , GLU , GLN , ASN , GLY , SER , ALA , THR , PRO , TYR , VAL , PHE , MET , TRP , LEU+ILE

Applications

Analysis of underivatized amino acids by LC/MS/MS

Column

ULTRON AF-HILIC-CD (PLSS)

Column Size

150 mm L × 2.0 mm I.D.

Mobile phase

A: 100 mM HCO2NH4(pH 3.0)/CH3CN = 10/90, B: 100 mM HCO2NH4(pH 3.0)/CH3CN = 70/30

Frow rate

0.36 mL/min

Column temp.

40°C

Injection volume

1.0 µL (5.0 µg/mL)

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