LC

Samples

Citric acid , Tartaric acid , Malic acid , Malonic acid , Succinic acid , Glycolic acid , Formic acid , Lactic acid , Acetic acid , Fumaric acid , Adipic acid , Pyroglutamic acid , Propionic acid , Acrylic acid , Pivalic acid , Methacrylic acid , Crotonic acid

Applications

Elution position of the aliphatic organic acid

Column

ULTRON PS-80H

Column Size

300 mm L × 8.0 mm I.D. + 300 mm L × 8.0 mm I.D.

Mobile phase

HClO4aq. (pH 2.1)

Frow rate

1.0 mL/min

Column temp.

60°C

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LC

Samples

Citric acid , Tartaric acid , Malic acid , Succinic acid , Lactic acid , Acetic acid , Pyroglutamic acid

Applications

Analysis of the soy sauce

Column

ULTRON PS-80H

Column Size

300 mm L × 8.0 mm I.D.

Mobile phase

HClO4aq. (pH 2.1)

Frow rate

1.0 mL/min

Column temp.

60°C

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LC

Samples

Aspartic acid , Glutamic acid , Serine , Glycine , Histidine , Arginine , Threonine , Alanine , Proline , Tyrosine , Valine , Methionine , Cysteine-cysteine , Isoleucine , Leucine , Phenylalanine , Lysine

Applications

Analysis of the PTC amino acid

Column

STR ODS-II

Column Size

150 mm L × 4.6 mm I.D.

Mobile phase

A: CH3COONa (pH 6.5)/CH3CN = 93/7, B: CH3CN/H2O = 4/1

Frow rate

1.0 mL/min

Column temp.

40°C

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LC

Samples

Nicotinic acid , Nicotinamide , Pantothenic acid , Pyridoxine , Riboflavin phosphate , Thiamine , Caffeine , Folic acid , Biotin , Riboflavin

Applications

The water-soluble vitamin (1)

Column

STR ODS-II

Column Size

150 mm L × 4.6 mm I.D.

Mobile phase

100 mM Phosphoric acid buffer(pH 2.1) + 0.8 mM Octane sodium sulphonate/CH3CN = 9/1

Frow rate

1.0 mL/min

Column temp.

40°C

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LC

Samples

Inosine 5'-monophosphate , Pyroglutamic acid

Applications

5'-IMP(Inosinic acid), Pyroglutamic acid

Column

ULTRON VX-ODS

Column Size

150 mm L × 4.6 mm I.D.

Mobile phase

20 mM Phosphoric acid buffer(pH 6.7)/CH3OH = 99/1

Frow rate

1.0 mL/min

Column temp.

40°C

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LC

Samples

Nicotinic acid , Nicotinic acid amide , Calcium pantothenate , Pyridoxine hydrochloride , Riboflavin phosphate , Thiamine , Folic acid , Biotin , Riboflavin

Applications

The water-soluble vitamin

Column

ULTRON VX-ODS

Column Size

150 mm L × 4.6 mm I.D.

Mobile phase

20 mM H3PO4/5 mM Sodium 1-hexanesulfonate = 91/9

Frow rate

1.0 mL/min

Column temp.

45°C

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LC

Samples

Nicotinamide , Pyridoxine , Isoascorbic acid , Riboflavin , Nicotinic acid , Ascorbic acid , Biotin , Pantothenic acid , Folic acid , Cyanocobalamin , Thiamine

Applications

Water-soluble vitamins

Column

ULTRON AF-HILIC-CD

Column Size

150 mm L × 2.0 mm I.D.

Mobile phase

A: 20 mM CH3CO2NH4(pH 6.8)/CH3CN = 10/90, B: 20 mM CH3CO2NH4(pH 6.8)/CH3CN = 70/30

Frow rate

0.25 mL/min

Column temp.

30°C

Injection volume

1.0 µL

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LC

Samples

(CYS)2 , LYS , ARG , HIS , ASP , GLU , GLN , ASN , GLY , SER , ALA , THR , PRO , TYR , VAL , PHE , MET , TRP , LEU+ILE

Applications

Analysis of underivatized amino acids by LC/MS/MS

Column

ULTRON AF-HILIC-CD (PLSS)

Column Size

150 mm L × 2.0 mm I.D.

Mobile phase

A: 100 mM HCO2NH4(pH 3.0)/CH3CN = 10/90, B: 100 mM HCO2NH4(pH 3.0)/CH3CN = 70/30

Frow rate

0.36 mL/min

Column temp.

40°C

Injection volume

1.0 µL (5.0 µg/mL)

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