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ULTRON HF-Phenyl

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What is ULTRON HF-Phenyl

  • The ULTRON HF-Phenyl column is capillary column that employ monolithic silica (hierarchical porous structure) bonded with phenyl groups for high-precision separation analysis.
  • Due to the π π interaction, the separation pattern is different from the other HF series.
  • Enables separation proteins that have not been enzymatically digested.
  • Enables suppression of carry-over by deactivation treatment on the silica surface.
  • Enables analysis of proteins under low-pressure condition.
  • It can be expected to searching unknown components and simultaneous analysis of various proteins.
  • The column inner diameter is 0.1 mm and can be used for nano flow LC system.
  • Suitable for LC/MS that requires high sensitivity detection.
  • The column is protected by a PEEK column jacket, making it easy to handle.

ULTRON HF-Phenyl Specifications

Column name ULTRON HF-Phenyl
Column type Phenyl
Column size
(length x inner diameter)
250 mm x 0.1 mm
700 mm x 0.1 mm
Applications Peptides, Intact proteins
Flow range Recommended range: 300~500 nL/min
pH range Recommended range: 2.0〜8.0
Recommended temperature range FEP sleeve: 30〜50°C (Max. temp. 50°C)
PEEK sleeve: 30〜60°C (Max. temp. 60°C)
Max allowable pressure FEP sleeve: 20.0 MPa
PEEK sleeve: 30.0 MPa

ULTRON HF-Phenyl Effect of column temperature (protein mixture)

In case the molecular weight is around 80 kDa, enables protein separation at temperatures close to body temperature.


ULTRON HF-Phenyl Column batch reproducibility (protein mixture)

Good reproducibility among column batches.


ULTRON HF-Phenyl Separation comparison with different column length (protein mixture)

Longer length columns achieve more defined separations.


ULTRON HF-Phenyl Effect of column temperature on mAb analysis

Enables analysis of mAb (145 kDa) at higher than 45℃.


ULTRON HF-Phenyl Carry-over of mAb

The column has showed more than 85% recovery at 50℃ column temperature.


ULTRON HF-Phenyl Analysis of positional isomers under water rich mobile phase

Due to π π interaction, the ULTRON HF-Phenyl column has ability to recognize positional isomers and different separation pattern from the ULTRON HF-ODS and HF-C4 column.
Shown below is analysis of positional isomers of cresol under water rich mobile phase condition with the ULTRON HF-Phenyl.


ULTRON HF-Phenyl Separation pattern of peptides comparison with other HF series columns

Shown below is comparison of peptides separation on the ULTRON HF series. The ULTRON HF-Phenyl and ULTRON HF-ODS columns are suitable for analysis of peptides. However, the peak profiles are different between the ULTRON HF-ODS and ULTRON HF-Phenyl.


ULTRON HF-Phenyl Fitting

Fitting Remarks
Standard specification
(FEP sleeve)
① 1/16” PEEK plug
② 1/16” O.D. x 0.35 mm I.D. x 40 mm FEP sleeve
③ 1/16” CTFE ferrule
④ 1/16” PEEK fitting
⑤ 1/32” PEEK column jacket
・ The above fittings corresponds to the VICI valve (ex. UltiMate 3000 RSLCnano system).
・ When installing the column to the system, tighten the column using a wrench (0.6 N.m as a guide).

Pressure resistant specification
(Inlet side: PEEK sleeve)
① 1/16” PEEK plug
② 1/16” O.D. x 0.41 mm I.D. x 40 mm PEEK sleeve
③ 1/16” PEEK High pressure ferrule
(Hybrid type of stainless steel and PEEK blended polymer)
④ 1/16” PEEK fitting
・ The above fittings corresponds to the VICI valve (ex. UltiMate 3000 RSLCnano system).
・ When tightening the ferrules use a wrench (1.4 N.m as a guide).
・ When installing the column to the system, tighten the column using a wrench (1.1 N.m as a guide).

The outer diameter of the capillary column is 0.38 mm. Please use suitable fittings (Sleeve, Ferrules, etc.) for your Nano Flow LC system. Normally, FEP sleeve fittings are shipped as a standard specification. Pressure resistant fittings (Inlet side: PEEK sleeve) are also available as option with additional cost.


Reference

PDF DownloadULTRON HF-Phenyl: Specifications and Prices

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